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Viking / A travelling laboratory

A scoop of soil. A very big question.

What can an experiment tell us about an unfamiliar world? Try the laboratory controls and think about more than one explanation for a result.

NASA Viking 1 Lander (1975-075C)NORAD: 8108

Viking 1 Lander

First successful Mars long-duration station · Thomas Mutch Memorial Station · Chryse Planitia

Resting Site

Chryse Planitia (The Golden Plain)

22.271° N, 312.049° E

Surface Lifespan

2,245 Sols

6.1 Earth years active

Power Source

2× SNAP-19 RTG

70 Watts (Plutonium-238)

HiRISE Imagery

PSP_001521_2025

Resolved from 280 km orbit

Lander Mass

572 kg

572 kg touchdown dry mass

The Greatest Biology Debate in Space Exploration History

Labeled Release (LR) Radioactive Gas Simulator

In 1976, Viking dropped a broth of nutrients tagged with radioactive Carbon-14 (¹⁴C) into Martian soil. The response shocked the scientific world.

Incubation Timeline: Day 3.5Active ¹⁴CO₂: 11,195 CPMControl (160°C): 18 CPM
Day 0 (Nutrient Injected)Day 1.5 (Initial Exponential Spike)Day 7 (2nd Inoculation)Day 10 (Saturation Plateau)
Radioactivity Rate Telemetry (Beta Emission Detector)Max: ~11,500 CPM
Raw Martian Regolith (Spike to 10,800+ CPM)Sterilized Soil Control (160°C, flat ~15 CPM)
The 2008 Resolution

The Perchlorate Salt Revolution

32 years later, Phoenix discovered perchlorate salts (ClO₄⁻) across Mars. Surface radiation turns perchlorates into superoxides.

When the nutrient broth was poured, the superoxides chemically ripped the amino acids apart into ¹⁴CO₂—explaining the spike completely without needing living biology!

Instrument: Labeled Release (LR)
PI: Gilbert V. Levin & Patricia Ann Straat

The 4 Onboard Soil Instruments

Labeled Release (LR)Gilbert V. Levin & Patricia Ann Straat

0.115 ml of carbon-14 labeled nutrient broth injected into 0.5 cm³ surface regolith

Outcome: Immediate, explosive evolution of radioactive 14CO2 gas (>10,000 counts per minute). When pre-heated to 160°C (control), the soil produced virtually zero gas.

Gas Chromatograph-Mass Spectrometer (GCMS)Klaus Biemann (MIT)

Soil heated in an oven to 50°C, 200°C, 350°C, and 500°C to volatilize organic molecules

Outcome: Found zero organic molecules above the instrument detection threshold of 1 part per billion (even less than Apollo Moon rocks).

Gas Exchange (GEX)Vance Oyama (NASA Ames)

Regolith exposed first to moisture (humidity) and then submerged in rich 'chicken soup' nutrient medium

Outcome: A sudden pulse of oxygen (O2) was released the moment humidity touched the soil, but adding liquid nutrient produced no sustained gas output.

Pyrolytic Release (PR)Norman Horowitz (Caltech)

Soil illuminated by simulated solar light in an atmosphere of 14CO2 and 14CO to test for photosynthesis

Outcome: A tiny amount of carbon was incorporated into the soil, but similar weak synthesis was observed in control tests held at high temperatures.

Look deeper: mission background

Authentic NASA Mission Context: Project Viking

In July 1976, Viking 1 became the first spacecraft to operate successfully on the surface of Mars. Its biology package was an engineering marvel: three miniature laboratories crammed into a one-cubic-foot automated container. The Labeled Release experiment gave a startling positive signal, but the lack of organic carbon detected by the GCMS puzzled scientists for decades until the 2008 Phoenix lander discovered oxidizing perchlorate salts in Martian soil.